Engineering the disulphide bond patterns of secretory phospholipases A2 into porcine pancreatic isozyme. The effects on folding, stability and enzymatic properties

European Journal of Biochemistry

Volumn 261, Issue 1, 1999, Pages 197-207

  Janssen, Marcel J W ^b   Verheij, Hubertus M ^b   Slotboom, Arend J ^a   Egmond, Maarten R ^b  

^a UTRECHT UNIVERSITY   (Netherlands)

^b NONE

Author keywords

Disulfide bond; Folding; Protein engineering; Secretory porcine pancreatic phospholipase A2; Stability

Indexed keywords

GUANIDINE; PANCREAS ENZYME; PHOSPHOLIPASE A2;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; DISULFIDE BOND; ENZYME ISOLATION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STABILITY; GENETIC ENGINEERING; NONHUMAN; PANCREAS CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FOLDING;

ANIMALS; BASE SEQUENCE; BINDING SITES; DISULFIDES; DNA PRIMERS; ENZYME STABILITY; ESCHERICHIA COLI; ISOENZYMES; MODELS, MOLECULAR; PANCREAS; PHOSPHOLIPASES A; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN FOLDING; SWINE;

EID: 0000226091     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00256.x     Document Type: Article

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