A rapid and efficient method for the isolation of differentially expressed Genes: Simplified differential display

Plant Biotechnology

Volumn 14, Issue 3, 1997, Pages 187-190

  Nakazono, Mikio ^a   Yoshida, Kaoru T ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000201623     PISSN: 13424580     EISSN: 13476114     Source Type: Journal    
DOI: 10.5511/plantbiotechnology.14.187     Document Type: Article

References (11)

1. Liang, P., Fardée, A. B., 1992. Science, 257: 967-971.
2. Welsh, J., Chada. K., Dalai, S.S., Cheng, R., Ralph, D., McClelland, ML, 1992. Nucleic Acids Res., 20: 4965-4970.
3. Yoshida, K. T., Naito, S., Takeda, G., 1994. Plant Cell Physiol., 35: 1003-1009.
4. Ausubel, P.M., Brent, R,. Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., Struhl, K. (eds), 1991. Current Protocols in Molecular Biology , Greene Publishing Associates & WileyInterscience, NY.
5. Watanabe, A., Price, C. A., 1982. Proc. Natl. Acad. Sci. USA, 79: 6304-6308.
6. Oligo(dT) primer instead of random primers is also available in the reaction of reverse transcription.
7. The thermal cycler that we used was a GeneAmp PCR System (Perkin Elmer, USA) or a THERMO PROCESSOR TR-100 (TAITEC). We reproducibly obtained the same bands from either thermal cycler.
8. Sambrook, J., Fritsch, E. F., Maniatis, T., 1989. "Molecular Cloning: A laboratory manual, 2nd edn." Cold Spring Harbor Press, Cold Spring Harbor, NY.
9. The "crush and soak" method that originally described by Maxam and Gilbert (1977) [11] is also available for isolation of DNA fragments from a polyacrylamide gel. This method has been described in detail in Molecular Cloning: A laboratory manual [8].
10. If necessary, cDNA fragments are reamplified by PCR and then are cloned into a plasmid vector.
11. Maxam, A. M., Gilbert, W., 1977. Proc. Natl. Acad. Sci. USA, 74: 560-564.