Partial male sterility in transgenic tobacco carrying antisense and sense PAL cDNA under the control of a tapetum-specific promoter

Plant and Cell Physiology

Volumn 37, Issue 2, 1996, Pages 215-222

  Matsuda, Narumi ^a   Tsuchiya, Tohru ^a   Kishitani, Sachie ^a   Tanaka, Yoshiyuki ^b   Toriyama, Kinya ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

Antisense inhibition; Male sterility; Phenyl alanine ammonia lyase (EC 4.3.1.5); Pollen development; Tapetum specific promoter; Transgenic tobacco

Indexed keywords

EID: 0000127194     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.pcp.a028934     Document Type: Article

References (43)

1. An, G., Ebert,. P.R., Mitra, A. and Ha, S.B. (1988) Binary vectors. In Plant Molecular Biology Manual. Edited by Gelvin, S.B. and Schilperoort, R.A. pp. A3/1-A3/19. Kluwer Academic Publishers, Dordrecht, Netherlands.
2. Beachy, R.N., Barton, K.A., Madison, J.T., Thompson, J.F. and Jarvis, N. (1979) The mRNAs that code for soybean seed proteins. In Genome Organisation and Expression in Higher Plants. Edited by Leaver, C. pp. 273-281. Plenum Publishing Corp., New York, U.S.A.
3. Cullen, D., Leong, S.A., Wilson, L.J. and Henner, D.J. (1987) Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hpt. Gene 57: 21-26.
4. Edwards, K., Cramer, C.L., Bolwell, G.P., Dixon, R.A., Schuch, W. and Lamb, C.J. (1985) Rapid transient induction of phenylalanine ammonialyase mRNA in elicitor-treated bean cells. Proc. Natl. Acad. Sci. USA 82: 6731-6735.
5. Elkind, Y., Edwards, R., Mavandad, M., Hedrick, S.A., Ribak, O., Dixon, R.A. and Lamb, C.J. (1990) Abnormal plant development and down-regulation of phenylpropanoid biosynthesis in transgenic tobacco containing a heterologous phenylalanine ammonia-lyase gene. Proc. Natl, Acad, Sci. USA 87: 9057-9061.
6. Gowri, G., Paiva, N.L. and Dixon, R.A. (1991) Stress responses in alfalfa (Medicago sativa L.) 12. Sequence analysis of phenylalanine ammonialyase (PAL) cDNA clones and appeatance of PAL transcripts elicitor-treated cell cultures and developing plants. Plant Mol. Biol. 17: 415-429.
7. Hall, T.C., Ma, Y., Buchbinder, B.U., Pyne, J.W., Sun, S.M. and Bliss, F.A. (1978) Messenger RNA for G1 protein of French bean seeds: cell-free translation and product characterization. Proc. Natl. Acad. Sci. USA 75: 3196-3200.
8. Horsch, R.B., Fry, J., Hoffmann, N., Neidermeyer, J., Rogers, S.G. and Fraley, R.T. (1988) Leaf disc transformantion. In Plant Molecular Biology Manual. Edited by Gelvin, S.B. and Schilperoort, R.A. pp. A5/1-A5/9. Kluwer Academic Publishers, Dordrecht, Netherlands.
9. Joos, H.J. and Hahlbrock, K. (1992) Phenylalanine ammonia-lyase in potato (Solanum tuberosum L.). Genomic complexity, structural comparison of two selected genes and modes of expression. Eur. J. Biochem. 204: 621-629.
10. Kehrel, B. and Wiermann, R. (1985) Immunochemical localization of phenylalanine ammonia-lyase and chalcone synthase in anthers. Plants 163: 183-190.
11. Kishitani, S., Yomoda, A., Konno, N. and Tanaka, Y. (1993) Involvement of phenytalanine ammonia-lyase in the development of pollen in broccoli (Brassica oleracea L.). Sex. Plant Reprod. 6: 244-248.
12. Koltunow, A.M., Truettner, J., Cox, K.H., Wollroth, M. and Goldberg, R.B. (1990) Different temporal and spatial gene expression patterns occur during anther development. Plant Cell 2: 1201-1224.
13. 1-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Mol. Gen. Genet. 204: 383-396.
14. Lois, R., Dietrich, A., Hahlbrock, K. and Schulz, W. (1989) A phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements. EMBO J. 8: 1641-1648.
15. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275.
16. Mo, Y., Nagel, C. and Taylor, L.P. (1992) Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen. Proc. Natl. Acad. Sci. USA 89: 7213-7217.
17. Moreno, R.M.B., Macke, F., Alwen, A. and Heverle-Bors, E. (1988) Insitu seed production after pollination with in-vitro-matured, isolated pollen. Plante 176: 145-148.
18. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
19. Murray, M.G. and Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucl. Acids. Res. 8: 4321-4325.
20. Napoli, C., Lemieux, C. and Jorgensen, R. (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of bomologous genes in trans. Plant Cell 2: 279-289.
21. Nelson, O.E. (1968) The waxy locus in maize. II. The location of the controlling element alleles. Genetics 60: 507-524.
22. Paul, W., Hodge, R., Smartt, S., Draper, J. and Scott, R. (1992) The isolation and characterization of the tapetum-specific Arabidopsis thaliana A9 gene. Plant Mol. Biol. 19: 611-622.
23. Pollak, P.E., Vogt, T., Mo, Y. and Taylor, L.P. (1993) Chalcone synthase and flavonol accumulation in stigmas and anthers of Petunia hybrida. Plant Physiol. 102: 925-932.
24. Rittscher, M. and Wiermann, R. (1983) Occurrence of phenylalanine ammonia-lyase (PAL) in isolated tapetum cells of Tulipa anthers. Protoplasma 118: 219-224.
25. Rittscher, M. and Wiermann, R. (1988) Studies on sporopollenin biosynthesis in Tulipa anthers. Sex. Plant Reprod. 1: 132-139.
26. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
27. Scott, R.J. (1994) Pollen exine - the sporopollenin enigma and the physics of pattern. In Molecular and Cellular Aspects of Plant Reproduction. Edited by Scott, R.J. and Stead, M.A. pp. 49-81. Cambridge University Press, UK.
28. Sheahan, J.J. and Rechnitz, G.A. (1992) Flavonoid-specific staining of Arabidopsis thaliana. Bio Techniques 13: 880-883.
29. Stanley, R.G. and Linskens, H.F. (1974) Pollen, Biology Biochemistry Management, p. 307. Springer-Verlag, Berlin.
30. Subramaniam, R., Reinold, S., Molitor, E.K. and Douglas, C.J. (1993) Structure, inheritance, and expression of hybrid poplar (Populus trichocarpa ×Populus deltoides) phenylalanine ammonia-lyase genes. Plant Physiol. 102: 71-83.
31. Tanaka, Y., Kojima, M. and Uritani, I. (1974) Properties, development and cellular-localization of cinnamic acid 4-hydroxylase in cut-injured sweet potato. Plant Cell Physiol. 15: 843-854.
32. Tanaka, Y., Matsuoka, M., Yamanoto, N., Ohashi, Y., Kano-Murakami, Y. and Ozeki, Y. (1989) Structure and characterization of a cDNA clone for phenylalanine ammonia-lyase from cut-injured roots of sweet potato. Plant Physiol. 90: 1403-1407.
33. Taylor, L.P. and Jorgensen R. (1992) Conditional male fertility in chalcone synthase-deficient petunia. J. Hered. 83: 11-17.
34. Tsuchiya, T., Toriyama, K., Ejiri, S and Hinata, (1994) Molecular characterization of rice genes specifically expressed in the anther tapetum. Plant Mol. Biol. 26: 1737-1746.
35. Tsuchiya, T., Toriyama, K., Yoshikawa, M., Ejiri, S. and Hinata, K. (1995) Tapetum-specific expression of the gene for an endo-β-1,3-glucan-ase causes male sterility in transgenic tobacco, Plant Cell Physiol. 36: 487-494.
36. van Blokland, R., van der Geest, N., Mol, J.N.M. and Kooter, J.M. (1994) Transgene-mediated suppression of chalcone synthase expression in Petunia hybrida results from an increase in RNA turnover. Plant J. 6: 861-877.
37. van der Krol, A.R., Lenting, P.E., Veenstra, J., van der Meer, I.M., Koes, R.E., Gerats, A.G.M., Mol. J.N.M. and Stuitje, A.R. (1988) An antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333: 866-869.
38. van der Krol, A.R., Mur, L.A., Beld, M., Mol, J.N.M. and Stuitje, A.R. (1990) Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell 2: 291-299.
39. van der Meer, I.M., Stam, M.E., van Tunen, A.J., Mol, J.N.M. and Stuitje, A.R. (1992) Antisense inhibition of flavonoid biosythesis in petunia anthers results in male sterility. Plant Cell 4: 253-262.
40. Vogt, T., Pollak, P., Tarlyn, N. and Taylor, L.P. (1994) Pollination- or wound-induced kaempferol accumulation in petunia stigmas enhances seed production. Plant Cell 6: 11-23.
41. Wiermann, R. (1970) Die Synthese von Phenylpropanen während der Pollenentwicklung. Planta 95: 133-145.
42. Ylstra, B., Basscher, J., Franken, J., Hollman, P.C.H., Mol. J.N.M. and van Tunen, A.J. (1994) Flavonols and fertilization in Petunia hybrida: localization and mode of action during pollen tube growth. Plant J. 6: 201-212.
43. Ylstra, B., Touraev, A., Moreno, R.M.B., Stöger, E., van Tunen, A.J., Vicente, O., Mol. J.N.M. and Heberle-Bors, E. (1992) Flavonols stimulate develpment, germination, and tube growth of tobacco pollen. Plant Physiol. 100: 902-907.