Towards the genetic engineering of triacylglycerols of defined fatty acid composition: Major changes in erucic acid content at the sn-2 position affected by the introduction of a 1-acyl-sn-glycerol-3-phosphate acyltransferase from Limnanthes douglasii into oil seed rape

Molecular Breeding

Volumn 2, Issue 2, 1996, Pages 133-142

  Brough, Clare L ^a   Coventry, Jane M ^b   Christie, William W ^c   Kroon, Johan T M ^a   Brown, Adrian P ^a,d   Barsby, Tina L ^b   Slabas, Antoni R ^a  

^a DURHAM UNIVERSITY   (United Kingdom)

^b Nickerson BIOCEM Ltd   (United Kingdom)

^c SCOTTISH CROP RESEARCH INSTITUTE   (United Kingdom)

^d JOHN INNES CENTRE   (United Kingdom)

Author keywords

1 acyl sn glycerol 3 phosphate acyltransferase; Brassica napus; Limnanthes douglasii; Trierucin

Indexed keywords

ENCODING (SYMBOLS); GENETIC ENGINEERING; GLYCEROL; OILSEEDS; PLANTS (BOTANY); UNSATURATED FATTY ACIDS;

1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE; ACID CONTENT; ACYLTRANSFERASES; BRASSICA NAPUS; ERUCIC ACID; FATTY ACID COMPOSITION; LIMNANTHES DOUGLASII; TRANSGENEIC PLANTS; TRIACYLGLYCEROLS; TRIERUCIN;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 0000106471     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00441428     Document Type: Article

References (33)

1. Bernerth R, Frentzen M: Utilisation of erucoyl-CoA by acyltransferases from developing seeds of Brassica napus (L.) involved in triacylglycerol biosynthesis. Plant Sci 67: 21-28 (1990).
2. Brown AP, Coleman C, Tommey AM, Watson MD, Slabas AR: Isolation and characterisation of a maize cDNA that complements a 1-acyl-sn-glycerol-3-phosphate acyltransferase mutant of Escherichia coli and encodes a protein which has similarities to other acyltransferases. Plant Mol Biol 26: 211-223 (1994).
3. Brown AP, Brough CL, Kroon JTM, Slabas AR: Identification of a cDNA that encodes a 1-acyl-sn-glycerol-3-phosphate acyltransferase from Limnanthes douglasii. Plant Mol Biol 29: 267-278 (1995).
4. Cao Y-Z, Oo K-C, Huang AHC: Lysophosphatidate acyltransferase in microsomes from maturing seeds of meadowfoam (Limnanthes alba). Plant Physiol 94: 1199-1206 (1990).
5. Christie WW: Gas Chromatography and Lipids. The Oily Press, Dundee (1989).
6. Coleman J: Characterization of Escherichia coli cells deficient in 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. J Biol Chem 265: 17215-17221 (1990).
7. Davies HM, Eriqat CA, Hayes TR: Utilization of laurate by the Kennedy pathway in developing seeds of Brassica napus expressing a 12:0-ACP thioesterase gene. In: Kader J-C, Mazliak P (eds) Plant Lipid Metabolism, pp. 503-505. Kluwer Academic Publishers, Dordrecht (1995).
8. Deblaere R, Bytebier B, De Greve H, Deboeck F, Schell J, Van Montagu M, Leemans J: Efficient octopine Ti plasmid derived vectors for Agrobacterium-mediated gene transfer to plants. Nucl Acids Res 13: 4777 (1985).
9. Hanke C, Wolter FP, Coleman J, Peterek G, Frentzen M: A plant acyltransferase involved in triacylglycerol biosynthesis complements an Escherichia coli sn-1-acylglycerol-3-phosphate acyltransferase mutant. Eur J Biochem 232: 806-810 (1995).
10. Hares W, Frentzen M: Properties of the microsomal acyl-CoA:sn-1-acyl-glycerol-3-phosphate acyltransferase from spinach (Spinacia oleracea L.) leaves. J Plant Physiol 131: 49-59 (1987).
11. Hares W, Frentzen W: Substrate specificities of the membrane bound and partially purified microsomal acyl-CoA:1-acylglycerol-3-phosphate acyltransferase from etiolated shoots of Pisum sativum (L.). Planta 185: 124-131 (1991).
12. Hitz WD, Yadav NS, Reiter RS, Mauvais CJ, Kinney AJ: Reducing polyunsaturation in oils of transgenic canola and soybean. In: Kader J-C, Mazliak P (eds) Plant Lipid Metabolism, pp. 506-508. Kluwer Academic Publishers, Dordrecht (1995).
13. Josefsson L-G, Lenman M, Ericson ML, Rask L: Structure of a gene encoding the 1.7S storage protein, napin, from Brassica napus. J Biol Chem 262: 12196-12201 (1987).
14. Kater MM, Koningstein GM, Nijkamp JJ, Stuitje A.R: cDNA cloning and expression of Brassica napus enoyl-acyl carrier protein reductase in Escherichia coli. Plant Mol Biol 17: 895-909 (1991).
15. Knauf VC: The application of genetic engineering to oilseed crops. Trends Biotechnol 5: 40-47 (1987).
16. Knutzon DS, Thompson GA, Radke SE, Johnson WB, Knauf VC, Kridl JC: Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Proc Natl Acad Sci USA 89: 2624-2628 (1992).
17. Löhden I, Frentzen M: Triacylglycerol biosynthesis in developing seeds of Tropaeolum majus L. and Limnanthes douglasii R. Br. Planta 188: 215-224 (1992).
18. Luddy FE, Barford R.A., Herb SF, Magidman P, Riemenschneider RW: J Am Oil Chem Soc 41: 693-696 (1964).
19. van de Loo FJ, Broun P, Turner S, Somerville C: An oleate 12-hydroxylase from Ricinus-communis L. is a fatty acyl desaturase homolog. Proc Natl Acad Sci USA 92: 6743-6747 (1995).
20. Mattson FH, Volpenhein RA: The specific distribution of fatty acids in the glycerides of vegetable fats. J Biol Chem 236: 1891-1894 (1961).
21. van der Meer IM, Brouwer M, Spelt CE, Mol JNM, Stuitje AR: The TACPyAT repeats in the chalcone synthase promoter of Petunia hybrida act as a dominant negative cis-acting module in the control of organspecific expression. Plant J 2: 525-535 (1992).
22. Moloney MM, Walker JM, Sharma KK: High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep 8: 238-242 (1989).
23. Ohlrogge J, Browse J: Lipid Biosynthesis. Plant Cell 7: 957-970 (1995).
24. Oo K-C, Huang AHC: Lysophosphatidate acyltransferase activities in the microsomes from palm endosperm, maize scutellum, and rapeseed cotyledon of maturing seeds. Plant Physiol 91: 1288-1295 (1989).
25. Phillips BE, Smith Jr, CR, Tallent WH: Glycerides of Limnanthes douglasii seed oil. Lipids 6: 93-99 (1970).
26. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York (1989).
27. Stacey J, Isaac PG: Protocols for nucleic acid analysis by nonradioactive probes. Meth Mol Biol 28: 9-15. Humana Press, Towota, NJ (1994).
28. Stymne S, Stobart AK: Triacylglycerol Biosynthesis. In: Stumpf PK, Conn EE (eds) The Biochemistry of Plants, vol. 9, pp. 175-214. Academic Press, New York (1987).
29. Taylor DC, MacKenzie SL, McCurdy AR, McVetty PBE, Giblin EM, Pass EW, Stone SJ, Scarth R, Rimmer SR, Pickard MD: Stereospecific analyses of seed triacylglycerols from high-erucic acid Brassicaceae: detection of erucic acid at the sn-2 position in Brassica oleracea L. genotypes. J Am Oil Chem Soc 71: 163-167 (1994).
30. Verwoert HGS, van der Linden KH, Nijkamp HJJ, Stuitje AR: Developmental specific expression and organelle targeting of the Escherichia coli fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase in transgenic rape and tobacco seeds. Plant Mol Biol 26: 189-202 (1994).
31. Voelker TA, Worrell AC, Anderson L, Fan C, Hawkins DJ, Radke SE, Davies HM: Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 257: 72-74 (1992).
32. Wen-jun S, Forde BG: Efficient transformation of Agrobacterium spp. by high voltage electroporation. Nucl Acids Res 17: 8385 (1989).
33. Yenofsky RL, Fine M, Pellow JW: A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure. Proc Natl Acad Sci USA 87: 3435-3439 (1990).