Thermal Unfolding of β-Lactoglobulin: Characterization of Initial Unfolding Events Responsible for Heat-Induced Aggregation

Journal of Agricultural and Food Chemistry

Volumn 45, Issue 11, 1997, Pages 4303-4308

  Prabakaran, Sakthivel ^a   Damodaran, Srinivasan ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Aggregation; Conformational change; Denaturation; Kinetics; Sulfhydryl disulfide interchange; Lactoglobulin

Indexed keywords

EID: 0000008540     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf970269a     Document Type: Article

References (22)

1. Anema, S. G.; McKenna, A. B. Reaction kinetics of thermal denaturation of whey proteins in heated reconstituted whole milk. J. Agric. Food Chem. 1996, 44, 422-428.
2. Chang, C. T.; Wu, C. S. C.; Yang, J. T. Circular dichroic analysis of protein conformation: Inclusion of the β-turns. Anal. Biochem. 1978, 91, 13-31.
3. Dannenberg, F.; Kessler, H. G. Reaction kinetics of the denaturation of whey proteins in milk. J. Food Sci. 1988, 53, 258-263.
4. de Wit, J. N.; Hontelez-Backx, E.; Adamse, M. Evaluation of functional properties of whey protein concentrates and whey protein isolates. 3. Functional properties in aqueous solution. Neth. Milk Dairy J. 1988, 42, 155-172.
5. El-Shazly, A.; Mahran, G. A.; Hofi, A. A. Kinetics of heat denaturation of β-lactoglobulin. Milchwissenschaft 1978, 33, 166-170.
6. Griffin, W. G.; Griffin, M. C. A. Time-dependent polydispersity of growing colloidal aggregates: predictions from dynamic light-scattering theory. J. Chem. Soc., Faraday Trans. 1993, 89, 2879-2889.
7. Griffin, W. G.; Griffin, M. C. A.; Martin, S. R.; Price, J. Molecular basis of thermal aggregation of bovine β-lactoglobulin A. J. Chem. Soc., Faraday Trans. 1993, 89, 3395-3405.
8. Harwalkar, V. R. Measurement of thermal denaturation of β-lactoglobulin at pH 2.5. J. Dairy Sci. 1980, 63, 1043-1051.
9. Harwalkar, V. R. Kinetic study of thermal denaturation of proteins in whey. Milchwissenschaft 1986, 46, 206-209.
10. Hillier, R. M.; Lyster, L. J. Whey protein denaturation in heated milk and cheese whey. J. Dairy Res. 1979, 46, 95-102.
11. Hoffmann, M. A. M.; Roefs, S. P. F. M.; Verheul, M.; Van Mil, P. J. J. M.; De Kruif, K. G. Aggregation of β-lactoglobulin studied by in situ light scattering. J. Dairy Res. 1996, 63, 423-440.
12. Huang, X. L; Catignani, G. L.; Swaisgood, H. E. Relative structural stabilities of β-lactoglobulin A and B as determined by proteolytic susceptibility and differential scanning calorimetry. J. Agric. Food Chem. 1994, 42, 1276-1280.
13. Iametti, S.; Cairoli, S.; De Gregori, B.; Bonomi, F. Modifications of high-order structures upon heating of β-lactoglobulin: Dependence on the protein concentration. J. Agric. Food Chem. 1995, 43, 53-58.
14. Kessler, H. G.; Beyer, H. J. Thermal denaturation of whey proteins and its effect in dairy technology. Int. J. Biol. Macromol. 1991, 13, 165-173.
15. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 1970, 227, 680-685.
16. Lyster, R. L. J. The denaturation of α-lactalbumin and β-lactoglobulin in heated milk. J. Dairy Res. 1970, 37, 233-243.
17. Manji, B.; Kakuda, Y. Thermal denaturation of whey proteins in skim milk. Can. Inst. Food Sci. Technol. 1986, 19, 163-166.
18. Papiz, M. Z.; Sawyer, L.; Eliopoulos, E. E.; North, A. C. T.; Findlay, J. B. C.; Sivaprasadarao, R.; Jones, T. A.; Newcomer, M. E.; Kraulis, P. J. The structure of β-lactoglobulin and its similarity to plasma retinol-binding protein. Nature 1986, 324, 383-385.
19. Park, K. H.; Lund, D. B. Calorimetric study of thermal denaturation of β-lactoglobulin. J. Dairy Sci. 1984, 67, 1699-1706.
20. Parris, N.; Purcell, J. M.; Ptashkin, S. M. Thermal denaturation of whey proteins in skim milk. J. Agric. Food Chem. 1991, 39, 2167-2170.
21. Roefs, S. P. F. M.; De Kruif, K. G. A model for the denaturation and aggregation of β-lactoglobulin. Eur. J. Biochem. 1994, 226, 883-889.
22. Shimada, K.; Cheftel, J. C. Texture characteristics, protein solubility, and sulfhydryl group/disulfide bond contents of heat-induced gels of whey protein isolate. J. Agric. Food Chem. 1988, 36, 1018-1025.